human normal cervical epithelial cells ect1 e6e7  (ATCC)

Journal: Cell Death Discovery

Article Title: CLC3 regulates V-ATPase to enhance lysosomal degradation and cisplatin resistance in cervical cancer cells

doi: 10.1038/s41420-025-02876-0

Figure Lengend Snippet: A Relative CLC3 mRNA expression levels in various CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi) compared to normal cervical epithelial cells (NCCs). * P < 0.05, ** P < 0.001. vs. NCCs. B Western blot analysis showing CLC3 protein expression in NCCs and different CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi), with GAPDH as a loading control. * P < 0.05, ** P < 0.001. vs. NCCs. C CLC3 mRNA expression in CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, and CaSKi) following CLC3 overexpression (CLC3 High ) or knockdown (CLC3 Low ) compared to control. * P < 0.05, ** P < 0.001. vs. Control. D Western blot analysis of CLC3 protein levels in CLC3 High , CLC3 Low , and control cells across the same cell lines, with quantification shown in the accompanying graphs. * P < 0.05, ** P < 0.001. vs. Control. E IC50 values for cisplatin, paclitaxel, and 5-FU in CLC3 High , CLC3 Low , and control groups in SiHa and CaSKi cells, showing CLC3 modulation’s effect on chemoresistance. * P < 0.05 vs. Control.

Article Snippet: CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi) and normal cervical epithelial cells (NCCs) were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO2.

Techniques: Expressing, Western Blot, Control, Over Expression, Knockdown